hestudyperiodislonger,hasbeengoingonformorethan10years,butworldwide,onlytwocasesofdrugsintothethreeclinicaltrials,5to6monthsintothetwodrugsinclinicaltrials;andtheoccurrenceofimprovedagronomictraits,theapplicationoflivestockproductioncanbegeneticallymodifiedstrainsoflivestockhasnotbeenborn.transgenicanimalsproducedlowefficiency,site-specificintegrationproblemscausedbyhighcostsandregulatoryfailure,aswellassexualreproductionoftransgenicanimalsappearsisolatedgenetictrait,itisdifficulttomaintainanexcellentwin-likeancestoristheconstraintsoftoday'spracticaluseoftransgenicanimalsofthemainreasonsfortheprocess. thesuccessofsomaticcellcloningfortheproductionoftransgenicanimalstostartanewrevolution,animalsomaticcellcloningtechnologytorapidlyenlargetransgenicanimalgermplasmgeneratedbytheeffectofprovidinginnovativetechnology.theuseofsimpletechnologytransfersomaticcellgenetransferoftheimplementationofgoals,thesourceofgermcellscanbeavoidedlivestockdifficultandinefficient.atthesametime,theuseofgeneticallymodifiedsomaticcellline,canbecarriedoutunderlaboratoryconditionsinthetransgenicpre-integratedandgenderpre-selection.nucleartransferintheformer,firstthepurposeofforeigngeneandmarkergene(suchasgenesandlagzantibioticneomycingene)fusiongeneintoculturedcells,themarkergenethroughtheselectionoftheperformanceoftransgenicandcloningofpositivecells,andthentothenuclear-positivecellstransplantedintoenucleatedoocytes,thelastproductionoftheanimalsshouldbeintheory100%ofthepositivetransgenicanimals.usingthismethod,schniekesuchas(bioreport,1997)havesuccessfullyobtainedsixtransgenicsheep,ofwhich3withhumanclottingfactorixgeneandmarkergene(neomycinresistancegene),threewithamarkergene,thepurposeofgeneintegrationwasashighas50%.cibelli(science,1997)usingthesamenucleartransfermethodtoobtainthreetransgeniccattle,confirmedthevalidityoftheact.fromthiswecanseethattoday'sanimalcloningtechnologyfortheapplicationofthemostimportantone,thatis,highvalue-addedtransgenicresearchanddevelopmentofclonedanimals. embryonicstemcells(es)isaformalladultcelltypesofthefullpotentialofstemcells.scientistshavebeentryingallkindsofstemcellsinducedtodifferentiateintospecifictypesoforganizations,toreplacethosedamagedtissue,suchastheimplantedcellstoproduceinsulinindiabeticpatients.scientistshavebeenabletomakepigescellsintobeatingcardiaccells,escellsistogenerateneuralcellsandmesenchymalcellsandmouseescellsdifferentiateintoendodermcells.theseresultsforthecellandtissuereplacementtherapyhasopeneduparoad.atpresent,scientistshavesuccessfullyisolatedhumanescells(thomson,suchas1998,science),andsomaticcellcloningtechnologyfortheproductionofapatient'sownescellspossible.thepatientswithsomaticcellnucleartransplantationintooocytestoformarecombinantembryocells,therecombinantembryostoblastocystinvitro,andthenisolatedfromtheblastocystwiththeescells,obtainedbytargetedescellstodifferentiateintospecificcelltypesrequired(suchasnervecells,musclecellsandbloodcells)forreplacementtherapy.nucleartransfermethodthattheultimategoalofstemcellsfortreatment,ratherthantheindividualtobecloned,scientistshavecalled"thetreatmentofcloning." cloningtechnologyintheapplicationofbasicresearchisalsoofgreatsignificance,whichtostudytheoccurrenceofgametesandembryos,differentiationofcellsandtissues,geneexpressionregulation,suchasnuclear-cytoplasmicinteractionmechanismfortheprovisionofatool. asanewresearchteaminpractice,thesuccessrateofclonedanimalsisstillverylow,wilmutstudygroupinnurturing"victoria"intheexperiment,theconvergenceofthe277eggcellnucleartransplantation,onlythe"victoria"inthislambsurvivalonly,thesuccessrateofonly0.36percent,atthesametimethefetalfibroblastcellsandembryoniccellsincloningexperimentswerealsothesuccessrateofonly1.7%and1.1%,evenusingthe"honolulu"technologytoalowerdegreeofdifferentiationoftheeggcumuluscellsasnucleardonor,thesuccessrateofonlyafewpercent. inaddition,someindividualsgivebirthtoshowlackofphysicalorlimitedimmunity.cloningincattleasanexample,japan,franceandothercountriestocultivateanumberofclonedcattlediedwithintwomonthsafterbirth;tofebruary2000,japanhasatotalof121somaticcellclonedcattlewasborn,butonly64survived.observationresultsshowthattheplacentalpartofcalfshelters,thebloodoxygencontentandtheconcentrationofgrowthfactorarelowerthannormal;somecalf'sthymus,spleenandlymphnodeshavenotbeennormaldevelopment;clonedanimalsthantheaverageprevalenceoffetalthetendencyoffastergrowthofanimals,whichmaybethecauseofdeath. eventhenormaldevelopmentofthe"victoria",hasalsobeenfoundtohavesignsofprematureaging.theendofchromosomescalledtelomeres,whichdeterminesthenumberofcellscanbesplit:eachwillsplitthetelomereshortening,whiletelomeredepletionwhencellslosttheabilitytosplit.in1998,scientistsfoundthat"victoria"inthecelltelomeresshorterthannormal,thatis,theircellsinabetterstateofsenescence.atthattime,thismaybeanadultsheepcellbycloning"dolly"causedtheadultcelltocellwiththeimprint,butthisinterpretationiscurrentlybei << 上一页 [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] ... 下一页 >>
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